C9848
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 | Immunofluorescence Double staining with Rabbit Anti-cfos (Cat. No. F7799) (red) at a 1:5,000 dilution, and Monoclonal Anti-Calbindin-D-28K (Cat. No. C9848) (green) at a 1:400 dilution, reveals neuronal activation in a sub-population of lateral septal cells. Developed with biotinylated Donkey Anti-Rabbit, followed by Streptavidin-Cy3 (Cat. No. S6402) and FITC-labeled Goat Anti-Mouse. From Dr. S. Shoham, Herzog Hospital Research Lab, Jerusalem, Israel. |
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C9848
Sigma
Monoclonal Anti-Calbindin-D-28K antibody produced in mouse
clone CB-955, ascites fluid
| MDL number: | MFCD00164553 |
Description
| Immunogen | bovine kidney calbindin-D |
| Specificity | The antibody does not react with other members of the EF-hand family, such as calbindin-D-9K, calretinin, myosin light chain, parvalbumin, S-100a, S-100b, S-100A2 (S100L) and S-100A6 (calcyclin). A weaker reactivity was observed with chicken calbindin-D-28K. |
| Application | The antibody may be used in immunohistochemistry (alcohol-, formalin-, and methacarn-fixed, paraffin-embedded, and frozen tissue sections). Enzymatic predigestion of formalin-fixed, paraffin-embedded sections improves immunohistochemical staining. |
Properties
| antibody form | ascites fluid |
| clone | CB-955 (monoclonal) |
| mol wt | antigen mol wt 28 kDa |
| contains | 15 mM sodium azide |
| species reactivity | goat, sheep, canine, guinea pig, bovine, pig, rat, human, feline, rabbit, mouse |
| application(s) | immunoblotting: 1:3,000 using MDBK cell extract |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using rat cerebellum sections | |
| indirect ELISA: suitable | |
| isotype | IgG1 |
| Gene Information | human ... CALB1(793) |
| rat ... Calb1(83839) | |
| mouse ... Calb1(12307) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Meyer, E.A., et al., Differences in Chemo- and Cytoarchitectural Features within Pars Principalis of the Rat Anterior Olfactory Nucleus Suggest Functional Specialization J. Comp. Neurol. 498, 786-795, (2007) |
Quinn, B., et al., Temporal gene expression profiling reveals CEBPD as a candidate regulator of brain disease in prosaposin deficient mice BMC Neurosci. 9, 76, (2008) Abstract |
